Bacillus Amyloliquefaciens: A Powerful Beneficial Bacteria

Mowlik

Last updated 2025-03-02

1.Introduction to Bacillus Amyloliquefaciens

Bacillus amyloliquefaciens is a Gram-positive, endospore-forming bacterium first isolated from soil in 1943 by Juichiro Fukumoto, known for its ability to produce liquefying α-amylase, which plays a crucial role in starch degradation. It is classified within the Bacillus subtilis species complex and is part of the operational group (OGBa), which includes related species such as Bacillus siamensis and Bacillus velezensis.

This bacterium is widely distributed in various environments, including soil, plants, and aquatic systems, and is recognized for its biotechnological applications, particularly as a plant growth-promoting bacterium (PGPB) due to its capabilities in nitrogen fixation, phosphate solubilization, and production of antimicrobial compounds and enzymes. Recent genomic studies have further highlighted its potential in agriculture and biocontrol, making it a subject of significant interest in biotechnological research.

2.What is Bacillus Amyloliquefaciens?

Bacillus amyloliquefaciens is a Gram-positive, endospore-forming bacterium that was first isolated from soil in 1943 by the Japanese scientist Juichiro Fukumoto. This bacterium is notable for its ability to produce α-amylase, an enzyme that liquefies starch, which is reflected in its name—'amyloliquefaciens'—indicating its starch-degrading capabilities.

Taxonomically, it belongs to the Bacillus subtilis species complex and is part of the operational group Bacillus amyloliquefaciens (OGBa), which includes other closely related species such as Bacillus siamensis and Bacillus velezensis. Members of this group are widely distributed in various environments, including soil, plants, and aquatic systems, and are recognized for their biotechnological applications.

They are known as plant growth-promoting bacteria (PGPB) due to their abilities to fix nitrogen, solubilize phosphate, and produce various enzymes and antimicrobial compounds that can inhibit the growth of pathogens. Recent advancements in genome mining have further revealed their potential in agricultural and biocontrol applications, making Bacillus amyloliquefaciens a significant focus of research in biotechnology.

3.Characteristics of Bacillus Amyloliquefaciens

Bacillus amyloliquefaciens exhibits several distinctive characteristics that contribute to its significance in various biotechnological applications. Here are some key features:

Gram-Positive and Endospore-Forming:

Bacillus amyloliquefaciens is classified as a Gram-positive bacterium, which means it retains the crystal violet stain used in the Gram staining procedure. It is also capable of forming endospores, allowing it to survive in harsh environmental conditions.

Morphological Traits:

This bacterium is rod-shaped and motile, utilizing peritrichous flagella for movement. These morphological traits are typical of many species within the Bacillus genus.

Taxonomic Classification:

Bacillus amyloliquefaciens is part of the Bacillus subtilis species complex and belongs to the operational group Bacillus amyloliquefaciens (OGBa). This group includes four species: B. amyloliquefaciens, B. siamensis, B. velezensis, and B. nakamurai.

Enzymatic Production:

One of the hallmark features of Bacillus amyloliquefaciens is its ability to produce various enzymes, including α-amylase, protease, lipase, cellulase, xylanase, pectinase, and others. These enzymes play crucial roles in biotechnological processes, such as improving feed digestibility in aquaculture.

Plant Growth-Promoting Abilities:

Members of the OGBa, including Bacillus amyloliquefaciens, are recognized as plant growth-promoting bacteria (PGPB). They can fix nitrogen, solubilize phosphate, and produce phytohormones, which enhance plant growth and health.

Antimicrobial Properties:

Bacillus amyloliquefaciens is known to produce antimicrobial compounds, including non-ribosomal peptides and polyketides, which can inhibit the growth of various pathogens. This characteristic makes it valuable in agricultural biocontrol applications.

Environmental Versatility:

This bacterium is widely distributed in various niches, including soil, plants, food, and water, showcasing its adaptability to different environments.

These characteristics make Bacillus amyloliquefaciens a significant organism in biotechnology, agriculture, and aquaculture, with ongoing research exploring its potential applications.

4.Overview of Bacillus Amyloliquefaciens in the Biotechnology Industry

Bacillus amyloliquefaciens is a versatile bacterium with significant applications in the biotechnology industry. Its unique characteristics and capabilities make it a valuable organism for various biotechnological processes. Here’s a detailed overview:

Enzyme Production:

One of the most notable features of Bacillus amyloliquefaciens is its ability to produce a wide range of enzymes, including α-amylase, protease, lipase, cellulase, xylanase, and pectinase. These enzymes are crucial in various industrial processes, such as food production, animal feed formulation, and bioremediation. For instance, α-amylase is widely used in the starch industry for converting starch into sugars.

Plant Growth Promotion:

Bacillus amyloliquefaciens is recognized as a plant growth-promoting bacterium (PGPB). It enhances plant growth through mechanisms such as nitrogen fixation, phosphate solubilization, and the production of phytohormones. These properties make it an essential component in sustainable agriculture, helping to improve crop yields and soil health.

Probiotic Applications:

In aquaculture, Bacillus amyloliquefaciens has shown potential as a probiotic. Studies have demonstrated that it can improve growth performance and feed utilization in fish, such as tilapia, and provide protection against pathogens in crustaceans like blue swimming crabs. This probiotic effect is attributed to its ability to enhance the immune response and overall health of aquatic organisms.

Biocontrol Agent:

The bacterium also acts as a biocontrol agent, producing antimicrobial compounds that inhibit the growth of various plant pathogens. This property is particularly beneficial in organic farming, where chemical pesticides are avoided. The use of Bacillus amyloliquefaciens can help manage diseases in crops, reducing the reliance on synthetic chemicals.

Commercial Products:

Several commercial products containing Bacillus amyloliquefaciens are available in the market, particularly for agricultural and aquaculture applications. For example, Ecobiol® Soluble Plus is a probiotic product formulated for use in poultry, swine, and aquaculture, demonstrating the bacterium's versatility in different sectors.

Future Prospects:

The ongoing research and genome mining of Bacillus amyloliquefaciens continue to reveal new biotechnological applications, including its potential for bioremediation of environmental pollutants and the production of bioactive compounds. This research is crucial for developing innovative solutions in agriculture, food production, and environmental management.

Bacillus amyloliquefaciens plays a multifaceted role in the biotechnology industry, contributing to agricultural productivity, aquaculture health, and environmental sustainability through its diverse applications and beneficial properties.

5.Antimicrobial Compounds Produced by Bacillus amyloliquefaciens

Bacillus amyloliquefaciens is recognized for its ability to produce a variety of antimicrobial compounds that play a crucial role in suppressing pathogens. Here’s a detailed overview of these compounds and their significance:

Types of Antimicrobial Compounds:

Members of the operational group Bacillus amyloliquefaciens (OGBa) produce several important antimicrobial compounds, including:

Non-ribosomal peptides:

These include surfactin, fengycin, bacillomycin-D, bacilysin, and bacillibactin. These compounds are known for their ability to disrupt cell membranes and inhibit the transfer of peptidoglycan precursors, which is vital for bacterial cell wall synthesis.

Polyketides:

This group includes bacillaene, macrolactin, and difficidin. Polyketides are biopolymers that exhibit various antifungal and antibacterial activities, making them valuable in the fight against resistant pathogens.

Mechanism of Action:

The antimicrobial compounds produced by Bacillus amyloliquefaciens function primarily by disrupting the integrity of microbial cell membranes. For instance, non-ribosomal peptides can inhibit the growth of various bacterial and fungal pathogens by interfering with their cellular processes.

Efficacy Against Pathogens:

Research has demonstrated the effectiveness of these compounds against a wide range of pathogens. For example, surfactins from B. velezensis have been shown to inhibit the growth of bacteria such as Bacillus cereus and Escherichia coli, as well as fungi like Alternaria solani and Fusarium oxysporum. Additionally, compounds like iturin A and bacillomycin F have exhibited strong antifungal activities against pathogens such as Magnaporthe grisea and Rhizoctonia solani.

Genomic Insights:

Genomic studies have revealed that B. amyloliquefaciens and its relatives possess gene clusters responsible for the biosynthesis of these antimicrobial compounds. This genomic information is crucial for understanding the potential of these bacteria in developing new antimicrobial agents.

Applications in Agriculture:

The antimicrobial properties of Bacillus amyloliquefaciens are leveraged in agriculture as biocontrol agents. By producing these compounds, the bacteria enhance plant resistance against various pathogens, thereby promoting healthier crop growth and reducing the need for chemical pesticides.

Bacillus amyloliquefaciens is a significant producer of antimicrobial compounds that are effective against a variety of pathogens. These compounds not only contribute to the bacterium's role in biocontrol and plant growth promotion but also hold promise for developing new therapeutic agents in the face of rising antibiotic resistance.

6.Uses of Bacillus amyloliquefaciens in Agriculture

Bacillus amyloliquefaciens (BA) is recognized as a highly effective agent in agriculture, particularly for its roles in biofertilization and biocontrol. Here are some key uses of BA in agricultural practices:

Biofertilizer:

BA enhances soil nutrient availability by improving nitrogen supply, solubilizing phosphate and potassium, and producing siderophores that chelate iron. This leads to better nutrient uptake by plants, promoting overall growth and yield.

Plant Growth Promotion:

BA acts as a plant growth-promoting rhizobacteria (PGPR). It produces phytohormones and volatile organic compounds (VOCs) that stimulate plant cell growth and root development, further enhancing nutrient absorption.

Stress Resistance:

BA enhances plant resistance to biotic stresses (like pathogens) and abiotic stresses (such as drought or salinity). It does this by competing for nutrients and niches, producing antimicrobial metabolites, and inducing systemic resistance in plants.

Diverse Applications:

BA has been applied to various crops, including rice, watermelon, cucumber, and tobacco, demonstrating its versatility in improving plant health and productivity across different agricultural systems.

Environmental Safety:

As a non-toxic and eco-friendly agent, BA serves as a sustainable alternative to chemical fertilizers and pesticides, contributing to environmentally friendly agricultural practices.

Bacillus amyloliquefaciens plays a multifaceted role in agriculture by promoting plant growth, enhancing nutrient availability, suppressing pathogens, and improving stress tolerance, all while being environmentally safe.

7.Role of Bacillus amyloliquefaciens in Plant Growth Promotion

Bacillus amyloliquefaciens (BA) plays a significant role in promoting plant growth through various mechanisms. Here are the key ways in which BA contributes to plant growth:

Enhancing Soil Nutrient Availability:

BA improves nitrogen supply, solubilizes phosphate and potassium, and produces siderophores that chelate iron, making these nutrients more available to plants.

Changing Soil Microbial Community:

The presence of BA can alter the soil microbial community, increasing the proportion of beneficial microbes while decreasing harmful pathogens. This shift enhances the overall availability of minerals and improves conditions for plant growth.

Production of Hormones and Volatile Organic Compounds (VOCs):

BA secretes hormones and VOCs that are crucial for plant cell growth and root development. These compounds help in promoting nutrient uptake by plants, leading to better growth.

Enhancing Resistance to Biotic Stresses:

BA helps plants resist biotic stresses from soil pathogens through several mechanisms:

It competes for niches and essential nutrients, thereby limiting pathogen growth.

BA produces antimicrobial metabolites, such as cyclic lipopeptides and polyketides, which directly inhibit pathogens.

It triggers the plant's innate immune response, known as induced systemic resistance (ISR), enhancing the plant's defensive capacity.

Inducing Tolerance to Abiotic Stresses:

BA can also promote plant growth by inducing tolerance to abiotic stresses. This is achieved through genetic, chemical, and physical changes in the host plant, which help it cope with adverse environmental conditions.

Field and Laboratory Evidence:

Numerous studies have demonstrated the effectiveness of BA strains in promoting plant growth. For example, specific BA strains have shown significant positive effects on the growth metrics of various plants, including increased fresh weight and root length.

Bacillus amyloliquefaciens serves as an excellent biofertilizer and biocontrol agent in agriculture, enhancing plant growth through improved nutrient availability, changes in microbial communities, hormone production, and increased resistance to both biotic and abiotic stresses.

8.Biocontrol Properties of Bacillus amyloliquefaciens Against Pathogens

Bacillus amyloliquefaciens (BA) exhibits several biocontrol properties that make it an effective agent against various plant pathogens. Here are the key mechanisms through which BA exerts its biocontrol effects:

Production of Antimicrobial Metabolites:

BA produces a range of antimicrobial metabolites, including non-ribosomal peptides, volatile organic compounds (VOCs), and defense enzymes. These substances help to antagonize and inhibit the growth of pathogens such as bacteria, fungi, nematodes, and viruses that threaten plant health and growth.

Competition for Nutrients and Ecological Niches:

BA effectively competes with pathogens for ecological niches and essential nutrients in the root zones. By colonizing these areas, BA can reduce the availability of resources for pathogens, thereby limiting their growth and establishment.

Siderophore Production:

One of the critical strategies employed by BA is the production of siderophores, such as bacillibactin. These compounds chelate iron, making it less available to pathogens, which rely on iron for their growth. This competitive advantage helps BA inhibit pathogens like Agrobacterium.

Induction of Systemic Resistance (ISR):

BA can trigger the plant's systemic resistance mechanisms, enhancing its defensive capacity against biotic stresses. This ISR is a crucial aspect of how BA helps plants withstand attacks from soil pathogens.

Biofilm Formation:

The ability of BA to form biofilms contributes to its biocontrol efficacy. Biofilms enhance the persistence of BA in the rhizosphere, allowing it to outcompete pathogens and establish a stable presence in the root zone.

Field and Laboratory Evidence:

Various studies have demonstrated the effectiveness of BA strains in controlling soil-borne pathogens, showcasing their potential as biocontrol agents in agricultural practices. These studies highlight the practical applications of BA in promoting plant health and reducing reliance on chemical pesticides.

In conclusion, Bacillus amyloliquefaciens possesses multiple biocontrol properties that enable it to effectively combat plant pathogens. Through the production of antimicrobial metabolites, competition for resources, and the induction of systemic resistance, BA serves as a promising agent for sustainable agricultural practices.

9.Industrial Applications of Bacillus amyloliquefaciens

Bacillus amyloliquefaciens is a versatile bacterium with numerous industrial applications due to its ability to produce various enzymes and bioactive compounds. Here are some key applications:

Amino Acids and Peptides Production:

B. amyloliquefaciens can synthesize a range of amino acids and peptides, which are valuable in the industrial sector for various applications, including food and pharmaceuticals.

GABA Biosynthesis:

The strain B. amyloliquefaciens EH-9 has been utilized for the biosynthesis of γ-aminobutyric acid (GABA), which is important for treating anxiety and sleep disorders. This strain was co-cultivated with germinated rice seeds, resulting in a supernatant rich in GABA that significantly enhanced collagen production when applied topically to mice.

Poly-γ-glutamic Acid (γ-PGA) Production:

B. amyloliquefaciens has shown potential in producing γ-PGA, a biodegradable polypeptide. Genetic engineering has been employed to enhance the yield of γ-PGA, resulting in strains that produce significantly higher amounts compared to the original strains.

Pectinase Production:

This bacterium is also involved in producing pectinases, which are crucial in the food industry. A study demonstrated the use of B. amyloliquefaciens TKU050 to produce pectinases from banana peels, reducing production costs and promoting environmentally friendly practices.

Enzyme Production for Biofuels:

The strain KUB29 has been identified for synthesizing thermostable enzymes that can be utilized in biofuel production, showcasing the potential of B. amyloliquefaciens in sustainable energy solutions.

Antimicrobial Compounds:

B. amyloliquefaciens produces various antimicrobial compounds, including surfactin and fengycin, which have applications in food safety by combating multi-drug-resistant pathogens. These compounds can be used in food preservation and as biocontrol agents in agriculture.

Fermentation Processes:

The bacterium's ability to produce enzymes such as amylase, protease, and lipase has led to its widespread use in food and beverage industries, contributing to processes like fermentation, baking, and brewing.

These applications highlight the significant industrial potential of Bacillus amyloliquefaciens, making it a valuable microorganism across various sectors.

10.Medical Applications of Bacillus amyloliquefaciens

Bacillus amyloliquefaciens has shown significant promise in various medical applications, demonstrating its potential to improve health outcomes in different contexts. Here are some key medical applications:

Obesity Management:

The strain B. amyloliquefaciens SC06 has been found to protect against high-fat diet-induced obesity in animal models. Mice fed an HFD along with SC06 exhibited reduced fat accumulation, lower obesity levels, and a slight decrease in insulin resistance, indicating its potential role in weight management and metabolic health.

Liver Protection:

SC06 treatment has also been associated with liver protection, as it decreased the secretion of inflammatory factors such as IL-6, TNF-α, and leptin. This suggests that B. amyloliquefaciens may help in modulating inflammation and improving liver health.

Stroke Recovery:

In a study involving ischemic stroke models, B. amyloliquefaciens strains CKJ730 and CKJ731 were shown to reduce neuronal cell death and improve neurological symptoms. The treatment enhanced glucose metabolism and insulin sensitivity, while also exhibiting anti-inflammatory effects by reducing proinflammatory cytokines.

Cancer Treatment:

A novel strain, B. amyloliquefaciens X030, produces a lipopeptide called bacillomycin Lb, which has demonstrated anticancer potential against various cancer cell lines. This highlights the bacterium's potential in cancer therapy.

Animal Health:

In veterinary medicine, B. amyloliquefaciens has been used to improve the health of grass carp by enhancing their microbiome composition and boosting immunity against infections. The strain was found to increase the expression of immune-related genes, thereby improving resistance to pathogens.

Infection Control:

The bacterium has been evaluated for its antimicrobial properties against nosocomial pathogens like Acinetobacter spp. A study showed that the cell-free supernatant of B. amyloliquefaciens B-1895 inhibited the growth and biofilm formation of these pathogens, suggesting its potential as a therapeutic agent in clinical settings.

Colitis Treatment:

In a TNBS-induced colitis model, camel milk enriched with B. amyloliquefaciens PBT-3 demonstrated beneficial effects by reducing colitis progression and modulating inflammatory cytokines, indicating its potential in treating inflammatory bowel diseases.

These applications underscore the versatility of Bacillus amyloliquefaciens in medical fields, ranging from metabolic health to infection control and cancer treatment.

11.Probiotic Applications of Bacillus amyloliquefaciens in Human Health

Bacillus amyloliquefaciens (B. amyloliquefaciens) has significant potential as a probiotic, contributing to various aspects of human health. Here are some key points regarding its applications:

Gastrointestinal Health:

B. amyloliquefaciens plays a crucial role in maintaining gastrointestinal health by producing essential nutrients and aiding in the digestion of complex food components. This helps improve overall digestive health in both humans and animals.

Resistance to Harsh Conditions:

The spore-forming nature of B. amyloliquefaciens allows it to withstand low pH levels and varying temperatures, making it resilient during gastric digestion. This characteristic enables it to reach the small intestine effectively, where it can exert its beneficial effects.

Probiotic Products:

Recent studies have shown that B. amyloliquefaciens is being incorporated into various probiotic products, including yogurt, kefir, tempeh, kimchi, and cheese. These products not only enhance gut health but also provide additional nutritional benefits.

Prebiotic Functions:

B. amyloliquefaciens also exhibits prebiotic properties by producing exopolysaccharides (EPS) such as levan and poly-γ-glutamic acid (γ-PGA). These compounds can enhance gut health by promoting the growth of beneficial gut bacteria and improving the intestinal barrier function.

Bioactive Compound Synthesis:

This bacterium is capable of synthesizing various bioactive compounds, including antioxidants and anti-inflammatory agents. These compounds can help combat oxidative stress and inflammation, contributing to overall health and potentially reducing the risk of chronic diseases.

Antimicrobial Properties:

B. amyloliquefaciens produces antimicrobial compounds that can inhibit pathogenic and food-spoiling microbes. This property not only enhances food safety but also supports gut health by reducing harmful bacteria in the digestive system.

Research and Future Applications:

Ongoing research continues to explore the full potential of B. amyloliquefaciens in functional food applications, emphasizing its role in health promotion and disease prevention. The findings suggest that this microbe could be a valuable addition to dietary supplements and functional foods aimed at improving human health.

B. amyloliquefaciens holds promise as a probiotic with multiple health benefits, including improved digestion, enhanced gut health, and the potential to combat various health issues through its bioactive compounds and antimicrobial properties.

12.Prebiotic Potential of Bacillus amyloliquefaciens

Bacillus amyloliquefaciens (B. amyloliquefaciens) exhibits significant prebiotic properties that contribute to health benefits for humans and animals. Here are the key aspects of its prebiotic potential:

Definition and Importance:

Prebiotics are typically nondigestible fibers and polysaccharides that promote the growth of beneficial gut bacteria. B. amyloliquefaciens is recognized for its ability to produce such compounds, enhancing gut health and overall well-being .

Production of Exopolysaccharides (EPS):

This bacterium synthesizes various EPS, including levan and poly-γ-glutamic acid (γ-PGA). These compounds serve as prebiotics, supporting the growth of beneficial microorganisms in the gut and improving intestinal health .

Hydrolysis of Complex Carbohydrates:

B. amyloliquefaciens can hydrolyze complex carbohydrates, such as inulin and rhamnogalacturonan, which are important prebiotic fibers. This hydrolysis process helps in breaking down these fibers into simpler forms that can be utilized by beneficial gut bacteria, thereby enhancing their growth and activity .

Health Benefits:

The prebiotic effects of B. amyloliquefaciens contribute to improved digestion, enhanced nutrient absorption, and a strengthened intestinal barrier. These benefits can lead to better overall health and a reduced risk of gastrointestinal disorders .

Applications in Food Products:

The prebiotic properties of B. amyloliquefaciens are being utilized in various food products, such as fermented foods. These applications not only improve the nutritional profile of the foods but also promote gut health through the action of prebiotics .

Research and Future Directions:

Ongoing research is focused on exploring the full potential of B. amyloliquefaciens as a prebiotic in functional foods. This includes studying its effects on gut microbiota composition and its role in preventing diseases related to gut health.

B. amyloliquefaciens demonstrates considerable prebiotic potential through its ability to produce beneficial compounds and enhance gut health. Its applications in food products and ongoing research highlight its importance in promoting human health and well-being.

13.Applications of Bacillus amyloliquefaciens in Food

Bacillus amyloliquefaciens (B. amyloliquefaciens) plays a crucial role in the food industry through various applications that enhance food quality and safety. Here are the key aspects of its applications in food:

Fermentation:

B. amyloliquefaciens is widely used in the fermentation of various food products. It can ferment different raw materials, including cereals, legumes, fruits, vegetables, dairy, meat, fish, and beverages. This fermentation process not only improves the flavor and texture of food but also increases its nutritional value and shelf life.

Hydrolysis of Macromolecules:

This bacterium is effective in hydrolyzing complex macromolecules such as proteins, carbohydrates, and fibers. The hydrolysis of proteins leads to the production of peptides, while carbohydrate hydrolysis breaks down polysaccharides into simpler sugars. This process is essential for enhancing the digestibility and nutritional profile of food products.

Synthesis of Bioactive Compounds:

B. amyloliquefaciens is known for its ability to synthesize bioactive compounds, including antimicrobial peptides and exopolysaccharides (EPS). These compounds not only contribute to the functional properties of food but also provide health benefits, such as antioxidant and anti-inflammatory effects.

Prebiotic and Probiotic Properties:

The bacterium serves as both a prebiotic and probiotic, promoting the growth of beneficial gut bacteria. Its prebiotic effects enhance gut health, while its probiotic properties help in maintaining a balanced gut microbiota, which is vital for overall health.

Antimicrobial Activity:

B. amyloliquefaciens produces antimicrobial compounds that can inhibit the growth of pathogenic and spoilage microorganisms in food. This antimicrobial activity is particularly important in extending the shelf life of food products and ensuring food safety.

Functional Food Applications:

The multifunctional properties of B. amyloliquefaciens make it a valuable ingredient in the development of functional foods. These foods are designed to provide health benefits beyond basic nutrition, such as improving gut health and reducing the risk of chronic diseases.

In summary, B. amyloliquefaciens is a versatile microbe with significant applications in the food industry, contributing to fermentation, hydrolysis, synthesis of bioactive compounds, and enhancing food safety and health benefits. Its role in functional foods further emphasizes its importance in modern food processing.

14.Conclusion

Bacillus amyloliquefaciens is a versatile bacterium with significant applications in agriculture, biotechnology, and human health. Its ability to promote plant growth, suppress pathogens, and contribute to industrial processes makes it a valuable resource for sustainable development.

15.FAQs

1. What is Bacillus amyloliquefaciens used for?

It is used in agriculture, biotechnology, and probiotic formulations.

2. Is Bacillus amyloliquefaciens safe for humans?

Yes, some strains are used as probiotics with beneficial effects.

3. How does Bacillus amyloliquefaciens help plants?

It improves nutrient absorption and fights plant pathogens.

4. Can Bacillus amyloliquefaciens replace chemical pesticides?

Yes, it serves as a natural biocontrol agent against harmful microbes.

5. What industries use Bacillus amyloliquefaciens?

Agriculture, biotechnology, food processing, and pharmaceuticals.